5-HT did not seem to affect either the mRNA level of the SERT or the protein transporter measured in either the membrane or the cell lysate. This inhibition was reversible and was not mediated by either the action of 5-HT2, 5-HT3 or 5-HT4 receptors, or by the intracellular second messengers, protein kinase C and cAMP. We measured serotonin transport, levels of mRNA expression and of the SERT protein after treating the cells with serotonin.Results: Serotonin treatment diminished hSERT activity in a concentration and period-dependent way by increasing the Kt value and reducing Vmax. These cells constitutively express the hSERT and have been shown to be an excellent model for the study of this protein. Aim: The aim of this study was to determine the effect of long-term serotonin (5-hydroxytryptamine, 5-HT) treatment on the human serotonin transporter (hSERT) function and its expression.Methods: This study was carried out in the enterocyte-like cell line Caco-2.
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